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human skin fibroblast  (ATCC)


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    ATCC human skin fibroblast
    Human Skin Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human skin fibroblast/product/ATCC
    Average 99 stars, based on 1832 article reviews
    human skin fibroblast - by Bioz Stars, 2026-05
    99/100 stars

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    Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into fibroblasts and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

    doi: 10.3389/fbioe.2026.1777175

    Figure Lengend Snippet: Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into fibroblasts and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.

    Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

    Techniques: Expressing

    Fibroblastic differentiation of hESC-MSCs. (A) Cellular morphology of hESC-Fbs and HSF. Scale bar = 100 μm. (B) Immunofluorescence analysis of VIM and CK5 expression in hESC-Fbs and HSF. VIM and CK5 were shown with green, nuclei were counterstained with blue. Scale bar = 100 μm. (C) qPCR assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). * p < 0.05 and ** p < 0.01 vs. hESC-MSCs. (D) ELISA assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). ** p < 0.01 vs. hESC-MSCs.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

    doi: 10.3389/fbioe.2026.1777175

    Figure Lengend Snippet: Fibroblastic differentiation of hESC-MSCs. (A) Cellular morphology of hESC-Fbs and HSF. Scale bar = 100 μm. (B) Immunofluorescence analysis of VIM and CK5 expression in hESC-Fbs and HSF. VIM and CK5 were shown with green, nuclei were counterstained with blue. Scale bar = 100 μm. (C) qPCR assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). * p < 0.05 and ** p < 0.01 vs. hESC-MSCs. (D) ELISA assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). ** p < 0.01 vs. hESC-MSCs.

    Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

    Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay

    Comprehensive Evaluation of TE-CuONPs Anticancer Activity and Apoptotic Mechanism in MCF-7 Breast Cancer Cells. Concentration-dependent cytotoxicity and mechanistic analysis. a , b MTT dose–response curves against MCF-7 breast cancer cells and normal human skin fibroblasts (HSF). c , d Representative phase-contrast micrographs (200 × magnification) of MCF-7 cells after 24 h treatment at respective IC₅₀ concentrations, showing c minimal morphological changes with TE treatment versus d characteristic apoptotic features with TE-CuONPs treatment, including cell shrinkage (red arrows), membrane blebbing (yellow arrows), chromatin condensation, and cellular detachment. e Flow cytometric scatter plots (FSC-H vs. SSC-H) demonstrating consistent cell populations across treatments (10,000 events gated per sample). ( f ) Schematic proposal for anticancer mechanisms. g Annexin V-FITC/propidium iodide (PI) dual-staining flow cytometric analysis

    Journal: Bioresources and Bioprocessing

    Article Title: Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity

    doi: 10.1186/s40643-026-01008-5

    Figure Lengend Snippet: Comprehensive Evaluation of TE-CuONPs Anticancer Activity and Apoptotic Mechanism in MCF-7 Breast Cancer Cells. Concentration-dependent cytotoxicity and mechanistic analysis. a , b MTT dose–response curves against MCF-7 breast cancer cells and normal human skin fibroblasts (HSF). c , d Representative phase-contrast micrographs (200 × magnification) of MCF-7 cells after 24 h treatment at respective IC₅₀ concentrations, showing c minimal morphological changes with TE treatment versus d characteristic apoptotic features with TE-CuONPs treatment, including cell shrinkage (red arrows), membrane blebbing (yellow arrows), chromatin condensation, and cellular detachment. e Flow cytometric scatter plots (FSC-H vs. SSC-H) demonstrating consistent cell populations across treatments (10,000 events gated per sample). ( f ) Schematic proposal for anticancer mechanisms. g Annexin V-FITC/propidium iodide (PI) dual-staining flow cytometric analysis

    Article Snippet: MCF-7 breast adenocarcinoma cells (ATCC HTB-22) and normal human skin fibroblasts (HSF, CRL-2522) were obtained from Nawah Scientific (Cairo, Egypt) and cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO2.

    Techniques: Activity Assay, Concentration Assay, Membrane, Staining